PART 3 - SPECIFICATION FOR ENVIRONMENTAL TESTING FOR APPROVAL OF BALLAST WATER MANAGEMENT SYSTEMS
3.1 The electrical and electronic sections of BWMS in the standard production configuration should be subjected to the programme of environmental tests set out in this specification at a laboratory approved for the purpose by the Administration or by the competent authority of the manufacturer's home country.
3.2 Evidence of successful compliance with the environmental tests below should be submitted to the Administration by the manufacturer together with the application for type approval.
Test specification details
3.3 Equipment should operate satisfactorily on completion of each of the operating environment tests listed below.
3.4 A resonance search should be made over the following ranges of oscillation frequency and amplitude:
.1 2 to 13.3 Hz with a vibration amplitude of 1 mm; and
.2 13.2 to 80 Hz with an acceleration amplitude of 0.7 g.
This search should be made in each of the three orthogonal planes at a rate sufficiently low to permit resonance detection.
3.5 The equipment should be vibrated in the above mentioned planes at each major resonant frequency for a period of two hours.
3.6 In the absence of any resonant frequency, the equipment should be vibrated in each of the planes at 30 Hz with an acceleration of 0.7 g for a period of two hours.
3.7 After completion of the tests specified in paragraph 3.5 or 3.6 a search should again be made for resonance and there should be no significant change in the vibration pattern.
3.8 Equipment that may be installed in exposed areas on the open deck, or in an enclosed space not environmentally controlled should be subjected, for a period of not less than two hours, to:
.1 a low temperature test at -25℃; and
.2 a high temperature test at 55℃.
3.9 Equipment that may be installed in an enclosed space that is environmentally controlled including an engine-room, should be subjected, for a period of not less than two hours, to:
.1 a low temperature test at 0℃; and
.2 a high temperature test at 55℃.
3.10 At the end of each of the tests referred to in the subparagraphs above, the equipment should be switched on and it should function normally under the test conditions.
3.11 Equipment should be left switched off for a period of two hours at a temperature of 55℃ in an atmosphere with a relative humidity of 90%. At the end of this period, the equipment should be switched on and should operate satisfactorily for one hour under the test conditions.
Tests for protection against heavy seas
3.12 Equipment that may be installed in exposed areas on the open deck should be subjected to tests for protection against heavy seas in accordance with 1P 56 of publication IEC 529 or its equivalent.
Fluctuation in power supply
3.13 Equipment should operate satisfactorily with:
.1 a voltage variation of +/- 10% together with a simultaneous frequency variation of +/- 5%; and
.2 a transient voltage of +/- 20% together with a simultaneous frequency transient of +/- 10%, with a transient recovery time of three seconds.
3.14 The BWMS should be designed to operate when the ship is upright and when inclined at any angle of list up to and including 15°either way under static conditions and 22.5°under dynamic conditions (rolling) either way and simultaneously inclined dynamically (pitching) 7.5°by bow or stern. The Administration may permit deviation from these angles, taking into consideration the type, size and service conditions of the ship and operational functioning of the equipment. Any deviation permitted is to be documented in the Type Approval Certificate.
Reliability of electrical and electronic equipment
3.15 The electrical and electronic components of the equipment should be of a quality guaranteed by the manufacturer and suitable for their intended purpose.
PART 4 - SAMPLE ANALYSIS METHODS FOR THE DETERMINATION OF BIOLOGICAL CONSTITUENTS IN BALLAST WATER
Sample processing and analysis
4.1 Samples taken during testing of BWMS are likely to contain a wide taxonomic diversity of organisms, varying greatly in size and susceptibilities to damage from sampling and analysis.
4.2 When available, widely accepted standard methods for the collection, handling (including concentration), storage, and analysis of samples should be used. These methods should be clearly cited and described in test plans and reports. This includes methods for detecting, enumerating, and identifying organisms and for determining viability (as defined in these Guidelines).
4.3 When standard methods are not available for particular organisms or taxonomic groups, methods that are developed for use should be described in detail in test plans and reports. The descriptive documentation should include any experiments needed to validate the use of the methods.
4.4 Given the complexity in samples of natural and treated water, the required rarity of organisms in treated samples under Regulation D-2, and the expense and time requirements of current standard methods, it is likely that several new approaches will be developed for the analyses of the composition, concentration, and viability of organisms in samples of ballast water. Administrations/Parties are encouraged to share information concerning methods for the analysis of ballast water samples, using existing scientific venues, and papers distributed through the Organization.
4.5 Sample analysis is meant to determine the species composition and the number of viable organisms in the sample. Different samples may be taken for determination of viability and for species composition.
4.6 Viability of an organism can be determined through live/dead judgement by appropriate methods including, but not limited to: morphological change, mobility. staining using vital dyes or molecular techniques.
4.7 A treatment test cycle should be deemed successful if:
.1 it is valid in accordance with paragraph 220.127.116.11 or 2.3.35 as appropriate;
.2 the average density of organisms greater than or equal to 50 micrometres in minimum diameter in the replicate samples is less than 10 viable organisms per cubic metre;
.3 the average density of organisms less than 50 micrometres and greater than or equal to 10 micrometres in minimum diameter in the replicate samples is less than 10 viable organisms per millilitre;
.4 the average density of Vibrio cholerae (serotypes O1 and O139) is less than 1 cfu per 100 millilitres, or less than 1 cfu per 1 gramme (wet weight) zooplankton samples;
.5 the average density of E. coli in the replicate samples is less than 250 cfu per 100 millilitres; and
.6 the average density of intestinal Enterococci in the replicate samples is less than 100 cfu per 100 millilitres.
4.8 It is recommended that a non-exhaustive list of standard methods and innovative research techniques be considered4.
4 Suggested sources may include but not be limited to:
.1 The Handbook of Standard Methods For the Analysis of Water and Waste Water
.2 ISO standard methods
.3 UNESCO standard methods
.4 World Health Organization
.5 American Society of Testing and Materials (ASTM) standard methods
.6 U.S. EPA standard methods
.7 Research papers published in peer-reviewed scientific journals
.8 MEPC papers.