Analysis of Genes Encoding ATP Synthase from a Psychrophilic Marine Bacterium, Vibrio sp. Strain ABE-1
Yasuhiro TAKADA*a, Shigeharu TAKIYAb, and Noriyuki FUKUNAGAa
a Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060, Japan
b Research Center for Molecular Genetics, Hokkaido University, Sapporo 060, Japan
An 255bp of DNA fragment was amplified by PCR with synthetic oligonucleotides as primers, which were designed from DNA sequences of the highly homologous region in, β subunits of Na+ -translocating ATPase from Propionigenium modestum and H+-translocating ATPases from Escherichia coli and a marine bacterium, Vibrio alginolyticus. By screening of the Vibrio ABE-1 genomic library with the PCR product as a probe, almost all genes encoding the ATPase of this bacterium were isolated and cloned. These genes formed a cluster and the gene order was the same as those of other bacteria described previously. The deduced amino acid sequence of the Vibrio ABE-1 ATPaseβ subunit was 86%, 77%, and 62% identical to those from E. coli, V. alginolyticus, and P. modestum, respectively. On the other hand, the deduced amino acid sequence of the Vibrio ABE-1 ATPase c subunit showed lower homology to those from the three bacteria (61%, 66%, and 27% identity, respectively).
1. Takada, Y., Fukunaga, N., and Sasaki, S. (1991) FEMS Microbiol. Lett., 82, 225-228.
2. Harashima, A., Takada, Y., Fukunaga, N., and Sasaki, S. (1993) Biosci. Biotech. Biochem., 57, 973-977.
3. Harashima, A., Takada, Y., and Fukunaga, N. (1996) Biosci. Biotech. Biochem., 60, 1324-1330.
