6 to 8 (95℃). It retained more than 80% activity after treatment at 95℃ for 3 hours. More than 50% activity was retained up to 24 hours at 95℃ in the presence of 1% SDS. Remaining activity after treatment at 95℃ for 1 hour with 50% of acetonitrile or 6.4 M urea was more than 80% and 70%, respectively. These data strongly suggest that protease PFUS has a great advantage suitable for industrial use.
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