homology (68.3% aa identity) to the isoleucyl-tRNA synthetase gene from Thermotoga maritima. This gene is used in the taxonomic classification of microorganisms.
The pullulanase was amplified by PCR and cloned into 3 E. coli vectors with different promoters (trc and PL) and enhancing elements. These constructs were transformed into a number of E. coli host strains and expression levels using the different systems were compared. These results and the results from sequence alignments will be presented.
1. Koch, R., Canganella, F., Hippe, H., Jahnke, K.D., and Antranikian, G. (1997) Appl. Environ. Microbiol., 63,1088-1094.
2. Antranikian, G. and Jφrgensen, P.L. (1992) European Patent EP 0578672 B1.
