Sequencing and Analysis of the Sulfolobus solfataricus P2 Genome
Christoph W. SENSEN*a,b, Robert L. CHARLEBOISb,c, W. Ford DOOLITTLEb,d, Michel DUGUETe, Terry GAASTERLANDbb,f,g, Roger GARRETTh, John VAN DER OOSTi, and Mark A. RAGANa,b
a National Research Council of Canada, Institute for Marine Biosciences, Canada
b Canadian Institute for Advanced Research, Program in Evolutionary Biology
c University of Ottawa, Canada
d Dalhousie University, Department of Biochemistry, Canada
e Universite Paris Sud, Institut de Genetique et Microbiologielaboratoire d'enzymologie des acides nucleiques, France
f Argonne National Laboratory, Mathematical and Computerscience, U.S.A
g University of Chicago, U.S.A., Department of Computerscience
h Copenhagen University, Denmark, Institute of Molecular Biology
i Wageningen Agricultural University, Department of Microbiology, The Netherlands
We are sequencing the entire 3 Mbp Sulfolobus solfataricus P2 genome as an international team effort. Sequencing is done on mapped cosmid and lambda clones. By the time of the conference, more than 80% of the Sulfolbus genome should be in the hands of the research team. Large contigs of several hundred kilobasepairs in size are starting to emerge. With most of the Sulfolobus ORFs now available, we can draw many specific and general conclusions about the organization and gene content of this archaeal genome. We will summarize results on gene content, repeat structure and comparative genomics derived from the analysis of the Sulfolobus genome.
