fragments derived from the HB27 genome. These fragments were used to transform HB27. Genomic analysis was carried out for the KmR transformants thus obtained. The KmR gene cassette existed at the intended sites. We have also succeeded in integrating pTT27 into the chromosome. These results indicated that genomic engineering is possible in T. thermophilus. Many feasible applications of the genomic engineering of T. thermophilus will be discussed.
1. Yokoyama et al. (1996) Arch. Microbiol., 165, 342-345.
2. Hoshino et al. (1994) FEMS Microbiol. Lett., 117, 175-180.
3. Hoshino et al. (1993) J. Ferment. Bioeng., 76, 276-279.
4. Maseda & Hoshino (1995) FEMS Microbiol. Lett., 128, 127-134.
5. Maseda & Hoshino (1996) J. Ferment. Bioeng., 82, 525-530.
6. Tabata & Hoshino (1996) Microbiology, 142, 401-410.
