P-3-05-11
THE EFFECT OF INTRAARTICULAR EXOGENOUS HYALURONIC ACID INJECTION ON THE HEALING OF TRAUMATIC CARTILAGE DAMAGE (An Experimental Study)
Nihat Tosun, Serdar Ugras, Fuat Akpinar (Y Yil University, Van, Turkey)
Objective: To examine the effect of intraartricular exogen hyaluronic acid injection on the regeneration of damaged cartilage.
Methods: Experiments were carried on the knee cartilage of 16 crossbred rabbits weighting 1.5 to 2kgs, cartilage damage of 0.5 cm was made by arhrotomy on the anterolateral side of lateral condyl of femur. Then 0.3mm hyaluronic acid was injected intraartricularly. The other knee which was damaged similarly served as control. No drug was injected after the damage to this knee. The surface of the damaged cartilage was macroscopically examined after sacrificing the rabbits and biopsies were obtained. Biopsy materials were evaluated histopathologically.
Results: Macroscopic examination revealed cartilage formation in some parts all the defect. Chondroblastic proliferation was seen in the histopathologic examination of both study and control groups which was more noticeable m some of the samples However chondroblastic proliferation was not noticeably different between these groups.
Conclusion: Hyaluronic acid injection was not so effective in the healing of traumatic cartilage damage as reported in the healing of degenerative cartilage disease.
Key Words: Hyaluronic Acid, Cartilage healing, Intra-articular injection.
P-3-05-12
EFFECT OF INTRAARTICULAR EXOGENOUS HYALURONIC ACID ON SYNOVIAL TISSUE (AN EXPERIMENTAL STUDY)
Nihat Tosun, Serdar Ugras, Fuat Akpmar, Ibrahim Tekeoglu, Ali Dogan, Metin Karakok
(Yuzuncu Yil University, Van, Turkey)
Purpose: We aimed to determine whether the effect of hyaluronic acid on synovial tissue was positive or negative.
Method: In this study, 12 native rabbits were used. Weight of the rabbits changed between 1.5 to 2kg, and all of them were healthy. 0.3 milliliter hyaluronic acid was injected intraarticularly in their right knee joint, secondly rejection was made on the fifth day m the same way. Left knee joints were washed by physiologic serum and no injection was performed, and accepted as control group. Three rabbits were excluded from this study, because two of them died due to joint infection, one rabbit died due to unknown reason. Nine rabbits were scarified after five weeks follow-up and synovial biopsies were obtained. Biopsy materials were evaluated histopathologically.
Result: Macroscopically, abnormal change was not observed in joints of nine rabbits. Histopathologically, no abnormal change was seen m synovial tissue of eight nine rabbits while numerous eosinophil leukocyte infiltration consistent with hypersensitivity reaction was seen in synovial tissue of one rabbit. There was not systemic hypersensitivity findings in this rabbit.
Conclusion: We concluded that intraarticular exogenous hyaluronic acid rejection did not have any negative effect on synovia, but local hypersensitivity reaction may rarely be seen on synovia.
Key words: Hyaluronic acid, synovial tissue, intraarticular injection.
