September 21 (Saturday):

Arrival at NARITA airport and Dr. Masashi Kirinoki,

Dr. Jun Matsumoto took me to Tochigi, Dockkyo University to meet Prof. Hajime Matsuda and Dr. Yuichi Chigusa.

　

September 22-23 (Saturday & Monday): Settle down

　

September 24-28 (Tuesday to Saturday):

・ Arrangement the schedule (Prof. Hajime Matsuda)

・ Digestion mice to exam the egg of S. mekongi in Liver and Intestine of mice.

・ How to collect egg of S. mekongi from liver

・ How to make the miracidum infected to intermediate host snail

・ How to collect cercaria from the snail Niotricula aperta infected

・ How to make the cercaria infected to final host (mice)

・ How to extract egg of S. mekongi from intestine mice infected to make antigen

　

September 29 (Sunday): Free

　

September 30 to October 04 (Monday to Friday):

・ Started learning on ELISA methode

・ Laboratory materials demonstration for Serology tests

・ Preparation Reagents for ELISA

・ Collected adult worms of S. mekongi and S. Japonicum from infected mice's by digestion method.

・ Preparation and purification of soluble egg antigen (SEA) from S. mekongi egg and S. Japonicum egg.

　

October 5-7 (Saturday to Monday):

・ Attended The 8^th Forrum Cheju Japan-Korea Parasitologists'Seminar at Otawa Club, Maebashi, Japan about New Challenge to Parasite immunology.

　

October 8-12 (Tuesday to Saturday):

・ Implement on ELISA with Antigen of S. Japonicum (Phlippine strain) and Antigen of S. mekongi

・ Collected Oncomelena snail in Obitsu River.

　

October 13-14 (Sunday and National holiday):

　

October 15-17 (Tuesday Thursday):

・ ELISA test continue

・ Practice of purifying egges of S. Japonicum from infected mice liver

　

October 18-20 (Friday to Sunday):

・ Attended The Parasitology Meeting in Niigata.

　

October 21-28 (Monday to Saturday):

・ Practice of Circum oval precipitate test (CPO) from infected mice of S. Japonicum and S. mekongi

・ Read COP slides for diagnosis and take photographs

・ Practice of miracidium hatching test and transmission to mice

・ Electrophoresis of antigen of S. Japonicum to differentiate the several types of antigens

・ ELISA practice continue-Sensitization of egg antigens of S. mekongi from infected mice in order to make antibody coated micro-plate wells.

　

October 29 (Tuesday)

・ ELISA for sera from Cambodia using antigens of S. Japonicum and S. mekongi.

　

October 30 (Wednesday):

・ COP slide reading

・ Stool examination using Formalin Detergent technique

　

October 31 (Thursday):

・ Ultrasonography for 13 patients

　

November 4-5 (Monday-Tuesday):

・ Collect Oncomelena snails in Kofu.

・ Visit Yamanashi Institute for Public Health

　

November 6 (Wednesday):

・ Visited Sasakawa Memorial Health Foundation and presented Shistosomiasis situation in Cambodia

・ Discuss the future plan of Schistosomiasis control in 2003.

　

November 7 (Thursday):

・ Summarized the study in Dokkyo university

　

November 8 (Friday):

・ Departure from Narita for Phnom Penh

　

　

1.Dokkyo University School of Medicine

2.Yamanashi Institute for Public Health, Mr. Noriaki KAjihara

3.Jichi University

4.Sasakawa Memorial Health Foundation